Flow cytometry data separated by time points.
Samples and cell populations were filtered independently for each time point.
Samples filter: excluded if median of viable cells fraction across all 5 tubes for a particular day is less than 0.7. In addition, subject 247 (Middle Eastern ethnicity) was removed as an outlier.
Cell population filter: excluded if 80% of samples have less than 20 cells
Data adjustment: Data (log10 transformed) were first pooled across all time-points, then were adjusted for the effects of age, gender and ethnicity. Adjustments were done for data with or without day0 subtraction (the latter for modeling responses).
Files:
dayX.raw : Raw data (% of parent population)
dayX.log10 : Log10 transformation was applied. Zeros were converted to 0.01 before the transformation.
dayX.corrected.demo : The data were adjusted for demographics (age, gender and ethnicity) by obtaining residuals from ANOVA model.
Regression constant coefficients/intercepts (for each population) were added back to the residual. All time points were processed together.
dayX-day0.log10 : day0 data (log10) were subtracted for each subject. Only samples and populations that passed filters for both time points were included.
dayX-day0.log10.corrected.demo : The dayX-day0 data for all time points were combined and adjusted for demographics by obtaining residuals from ANOVA model as above.